NODE provides several key features to help you work with marine genomic data:

• Explore projects, samples, analyses, features, and taxonomies
• Search across datasets using powerful query capabilities
• Submit your own data in standardized formats
• Download and reuse existing datasets
• Visualize taxonomic information with integrated visualization tools

We welcome your feedback to improve NODE. If you encounter any issues or have suggestions for new features, please let us know.

You can submit bug reports, feature requests, or general feedback through our GitHub issues page:

NODE GitHub Issues

When reporting bugs, please include:

• A clear description of the issue
• Steps to reproduce the problem
• What you expected to happen
• What actually happened
• Screenshots if applicable

Projects represent research initiatives or sampling campaigns. Each project contains multiple samples and may have associated analyses.

Key project information includes:

• Project name and description
• Principal investigator and collaborators
• Temporal and geographic scope
• Associated samples and analyses

Note: You can remove projects you have submitted. Removing a project will also remove any associated analyses.

Samples represent physical specimens or environmental samples collected during a project. They form the basis for subsequent analyses.

Sample data typically includes:

• Collection location and date
• Sample type and processing method
• Environmental context data
• Storage information

Analyses represent the results of processing samples through various methods such as DNA sequencing, PCR, or other molecular techniques.

Important information about analyses:

• Analyses are linked to projects
• You can add analyses to projects you did not submit
• You can see who the project belongs to when adding analyses
• You can view and remove your own analyses through the My Submissions Manager

Analysis data typically includes information about the sequencing method, bioinformatic processing, and taxonomic assignments.

Features represent unique DNA sequences (e.g., Amplicon Sequence Variants or ASVs) found in samples, typically representing distinct organisms.

Each feature includes:

• A unique identifier
• The DNA sequence
• Sequence length information
• Consensus taxonomic classification
• Prevalence across samples

Features provide the foundation for taxonomic classification and biodiversity assessment.

Taxonomies show the biological classification of organisms identified in your samples, from domain to species level.

The taxonomic outline image is sourced through PhyloPic, using GBIF Suggest API to match our taxonomy with PhyloPic's database. Images on PhyloPic are contributed by scientists and artists worldwide under various Creative Commons licenses.

If no image is displayed for a taxonomy, it could be due to:

• The taxonomy is unregistered in reference databases
• The taxonomy is a CLADE designation
• PhyloPic does not have an image for that taxonomy
• GBIF Suggest API did not return a matching taxonomy

To perform a basic search:

1. Enter keywords in the search bar
2. Select the category you want to search (Projects, Samples, etc.)
3. Use filters to narrow down results
4. Review the matching results

The search function looks for matches in titles, descriptions, metadata, and other relevant fields.

Here are some example queries to help you get started:

NODE follows community standards to ensure data quality, interoperability, and usability. Our data formats are designed with the following principles in mind:

• Darwin Core (DwC) for biodiversity data
• FAIR principles (Findable, Accessible, Interoperable, Reusable)
• Other relevant community standards

Standardized formats ensure that your data can be easily discovered, understood, and reused by other researchers.

The My Submissions Manager allows you to view, manage, and track your submitted data.

Note: You must be signed in to access the My Submissions Manager.

From the My Submissions Manager, you can:

• View your submitted projects and analyses
• Check submission status
• Edit or update submissions
• Remove your submissions

To submit a project, you'll need to prepare a metadata file following our template format.

The project submission process includes:

1. Preparing your project metadata
2. Filling out the submission form
3. Uploading your metadata file
4. Reviewing and confirming your submission

Required fields include project name, description, principal investigator, and collection dates.

All files must be in TSV format and follow the template structure exactly.

Analysis submissions require information about the analysis methods, results, and associated project.

The analysis submission process includes:

1. Selecting the associated project
2. Preparing your analysis data files
3. Filling out the analysis metadata form
4. Uploading your files
5. Reviewing and confirming your submission

Required fields include analysis type, sequencing method, and bioinformatic processing details.

All files must be in TSV format and follow the template structure exactly.